<p>RMF and HFF cells were either grown in the presence or absence of 50 μM LY294002 for 2 hours prior to infection and throughout the duration of infection. Cells were collected at 1, 3, 6 hpi, lysed, fractionated, immunoblotted, then probed for pAkt (S473) and total Akt. These results are representative of 3 independent experiments.</p
<p>PM (3×10<sup>6</sup>/ml) were stimulated for 1 h in the presence or absence (NS) of V<sub>H</sub>...
<p>Immunoblot analysis of total lysates from the Ba/F3 derived cell lines BY, BC, BYA and BCS. Cells...
<p><b>A.</b> ISG15+/+ or ISG15<b>−/−</b> macrophages were infected with VACV (WR strain, 60 PFU/cel)...
<p>RMF and HFF cells were grown in the presence or absence of LY294002 for 2 hours prior to infectio...
AbstractMany viruses activate the phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway, thereb...
<p>Serum-starved HEK293 cells were incubated with serum-free medium (−), medium containing 10% FBS (...
<p>Macrophages were treated or not treated with PI3K inhibitors and infected with <i>L. pneumophila<...
<p>(A) TMV activated Akt in NEC. NEC were incubated with TMV (50 µg/ml) for the indicated time perio...
(A) HEK 293 cells were transfected with HA-AKT together with HPV-16 E7 FLAG-HA, wildtype or 16 E7 S3...
<p>Fat body lysates isolated at the designated time points after infection were subjected to western...
(A) Immunoblotting of puromycin-labelled proteins in HepG2.215 control and HepG2.215-SENP3 K.D. cell...
<p><b>A</b>) EGFR and tyrosin kinases are important for ASFV uptake. Virus entry was measured by FAC...
Many viruses activate the phosphatidylinositol 3-kinase (PI3k)/Akt intracellular signaling pathway t...
<p>(A) HeLa cells were treated with control or Vps34 siRNA for 48 h. Cells were infected with wild t...
<p>HUVEC transfected with the indicated siRNAs were serum starved in M199 containing 0.5% FBS overni...
<p>PM (3×10<sup>6</sup>/ml) were stimulated for 1 h in the presence or absence (NS) of V<sub>H</sub>...
<p>Immunoblot analysis of total lysates from the Ba/F3 derived cell lines BY, BC, BYA and BCS. Cells...
<p><b>A.</b> ISG15+/+ or ISG15<b>−/−</b> macrophages were infected with VACV (WR strain, 60 PFU/cel)...
<p>RMF and HFF cells were grown in the presence or absence of LY294002 for 2 hours prior to infectio...
AbstractMany viruses activate the phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway, thereb...
<p>Serum-starved HEK293 cells were incubated with serum-free medium (−), medium containing 10% FBS (...
<p>Macrophages were treated or not treated with PI3K inhibitors and infected with <i>L. pneumophila<...
<p>(A) TMV activated Akt in NEC. NEC were incubated with TMV (50 µg/ml) for the indicated time perio...
(A) HEK 293 cells were transfected with HA-AKT together with HPV-16 E7 FLAG-HA, wildtype or 16 E7 S3...
<p>Fat body lysates isolated at the designated time points after infection were subjected to western...
(A) Immunoblotting of puromycin-labelled proteins in HepG2.215 control and HepG2.215-SENP3 K.D. cell...
<p><b>A</b>) EGFR and tyrosin kinases are important for ASFV uptake. Virus entry was measured by FAC...
Many viruses activate the phosphatidylinositol 3-kinase (PI3k)/Akt intracellular signaling pathway t...
<p>(A) HeLa cells were treated with control or Vps34 siRNA for 48 h. Cells were infected with wild t...
<p>HUVEC transfected with the indicated siRNAs were serum starved in M199 containing 0.5% FBS overni...
<p>PM (3×10<sup>6</sup>/ml) were stimulated for 1 h in the presence or absence (NS) of V<sub>H</sub>...
<p>Immunoblot analysis of total lysates from the Ba/F3 derived cell lines BY, BC, BYA and BCS. Cells...
<p><b>A.</b> ISG15+/+ or ISG15<b>−/−</b> macrophages were infected with VACV (WR strain, 60 PFU/cel)...