Additional file 3: Figure S3. of Immunolocation and enzyme activity analysis of Cryptosporidium parvum enolase

Western blot analysis of purified rCpEno. Purified rCpEno and induced pET-28a (+) plasmid transfected E. coli BL21 (DE3) competent cells were incubated with His-Tag mouse mAb (a) and C. parvum positive serum from cattle (b), respectively. Lane M: prestained protein ladder (Thermo Fisher Scientific). (TIF 902Â kb