Immunostaining of γ-H2AX signals in single, double and triple heterozygous mutant chromosomes at zygotene and pachytene stages.

<p>(A-H) The distribution of γ-H2AX on zygotene chromosomes showed no obvious differences among the eight genotypes examined. (I-P) The γ-H2AX distribution in pachytene chromosomes from single, double and triple heterozygous mutants. Left panels show the chromosome morphology following staining with 6-diamidino-2-phenylindole (DAPI), middle panels show γ-H2AX foci (red dots), and right panels merge the DAPI-stained images with the γ-H2AX foci images. (Q-R) The number of γ-H2AX foci in chromosomes from the eight genotypes at zygotene and pachytene in A-P. Scale bar: 5 μm. * p<0.05, ** p<0.01 (two-tailed Student’s <i>t</i>-test).