Number of chiasmata and AtMLH1 foci in double and triple heterozygous mutants.

<p>(A-E) Centromere-fluorescence <i>in situ</i> hybridization showing wild type (WT) and <i>atrad51</i>^<i>-/+</i> <i>atrad51c</i>^<i>-/+</i>, <i>atrad51</i>^<i>-/+</i> <i>atxrcc3</i>^<i>-/+</i>, <i>atrad51c</i>^<i>-/+</i> <i>atxrcc3</i>^<i>-/+</i> and <i>atrad51</i>^<i>-/+</i> <i>atrad51c</i>^<i>-/+</i> <i>atxrcc3</i>^<i>-/+</i> double and triple heterozygous mutant chromosome morphologies at diakinesis. Red dots indicate centromere signals. (F-J) Immunostaining of AtMLH1 foci (red dots) on WT, <i>atrad51</i>^<i>-/+</i> <i>atrad51c</i>^<i>-/+</i>, <i>atrad51</i>^<i>-/+</i> <i>atxrcc3</i>^<i>-/+</i>, <i>atrad51c</i>^<i>-/+</i> <i>atxrcc3</i>^<i>-/+</i> and <i>atrad51</i>^<i>-/+</i> <i>atrad51c</i>^<i>-/+</i> <i>atxrcc3</i>^<i>-/+</i> diakinesis chromosomes. (K) Number of chiasmata in WT, double and triple mutant chromosomes. (L) Number of AtMLH1 foci in WT, double and triple mutants. Scale bar: 5μm. * p<0.05, ** p<0.01 (one-tailed Student’s <i>t</i>-test).