<p><b>A)</b> Western blot analysis of HeLa protein lysates after siRNA transfection with siRNA Scrambled control, or siRNA FOXC1. As FOXC1 has a number of phosphorylation sites, a number of bands are observed at ~70kDa, indicated by a curly brace. Blots were probed for FOXC1, RAB3GAP1, RAB3GAP2, SNAP25 and α-Tubulin (loading control). Normalized and scaled values for <b>B)</b> RAB3GAP1 <b>C)</b> RAB3GAP2 and <b>D)</b> SNAP25. All Western blots were repeated at least three times. *<i>P</i>˂0.05, **<i>P</i>˂0.01, ***<i>P</i><0.001.</p
<p>Transactivation experiments with plasmid expressing FOXC1 (WT) or mutant FOXC1(p.S131L), and repo...
<p><b>(A and B</b>) siRNA against PAX3-FOXO1 (siPF) or control siRNA (siCONT) were transfected in RH...
<p>(A) EPCs were transfected with the indicated vectors. Cells were lysed and protein expression was...
<p>Western blot analysis using HeLa cell lysate, and cell media collected after transfection with pR...
<p><b>A)</b> Western blot analysis of intracellular (left) and extracellular (right) MYOC upon knock...
<p>qRT-PCR experiments using RNA isolated from HeLa cells transfected with scrambled siRNA. qPCR was...
<p>(<b>A</b>) Western-blot analyses to detect the indicated signaling molecules, using total cell ly...
<p>(<b>A</b>) HEK293 cells were co-transfected with HA-Foxp3 and Flag-SIRT1 and treated with 2 µM MG...
<p>(A) SIRT1 affects FOXA2 protein stability. HEK293T cells were transfected with FLAG-FOXA2 in the ...
<p>(<b>A</b>) HEK293 cells ectopically expressing HA-Foxp3 were transfected with Flag-SIRT1. Cell ly...
<p>(A) HepG2 cells were treated with 5 μM Cd for various time intervals. Cells were harvested and cy...
<p>Twenty five micrograms of cell lysates from INS-1 cells transfected with control siRNA or individ...
<p>(A) Over-expression of FOXO1 (lane 2) and FOXO3a (lane 3), as detected by western blotting, induc...
<p>(<b>A</b>) PCNA monoubiquitinylation and (<b>B</b>) RPA32 and H2AX phosphorylation analysis. HeLa...
<p>Note, the HNF4α served as a positive control while Actin was used as loading control. Human liver...
<p>Transactivation experiments with plasmid expressing FOXC1 (WT) or mutant FOXC1(p.S131L), and repo...
<p><b>(A and B</b>) siRNA against PAX3-FOXO1 (siPF) or control siRNA (siCONT) were transfected in RH...
<p>(A) EPCs were transfected with the indicated vectors. Cells were lysed and protein expression was...
<p>Western blot analysis using HeLa cell lysate, and cell media collected after transfection with pR...
<p><b>A)</b> Western blot analysis of intracellular (left) and extracellular (right) MYOC upon knock...
<p>qRT-PCR experiments using RNA isolated from HeLa cells transfected with scrambled siRNA. qPCR was...
<p>(<b>A</b>) Western-blot analyses to detect the indicated signaling molecules, using total cell ly...
<p>(<b>A</b>) HEK293 cells were co-transfected with HA-Foxp3 and Flag-SIRT1 and treated with 2 µM MG...
<p>(A) SIRT1 affects FOXA2 protein stability. HEK293T cells were transfected with FLAG-FOXA2 in the ...
<p>(<b>A</b>) HEK293 cells ectopically expressing HA-Foxp3 were transfected with Flag-SIRT1. Cell ly...
<p>(A) HepG2 cells were treated with 5 μM Cd for various time intervals. Cells were harvested and cy...
<p>Twenty five micrograms of cell lysates from INS-1 cells transfected with control siRNA or individ...
<p>(A) Over-expression of FOXO1 (lane 2) and FOXO3a (lane 3), as detected by western blotting, induc...
<p>(<b>A</b>) PCNA monoubiquitinylation and (<b>B</b>) RPA32 and H2AX phosphorylation analysis. HeLa...
<p>Note, the HNF4α served as a positive control while Actin was used as loading control. Human liver...
<p>Transactivation experiments with plasmid expressing FOXC1 (WT) or mutant FOXC1(p.S131L), and repo...
<p><b>(A and B</b>) siRNA against PAX3-FOXO1 (siPF) or control siRNA (siCONT) were transfected in RH...
<p>(A) EPCs were transfected with the indicated vectors. Cells were lysed and protein expression was...