Structure of rNm-ACPI and comparison with the MliC/PliC canonical fold.

<p><b>A)</b> Ribbon structure, showing the eight β-strands and disulphide bond. <b>B)</b> Topology diagram showing residue ranges in each β-strand, and the disulfide bond between Cys38 and Cys121 residues. <b>C)</b> Two orthogonal views of rNm-ACPI with the corresponding electrostatic surface in the same orientations below. <b>D)</b> Structural superposition of rNm-ACPI (blue) with PliC from <i>Brucella abortus</i> (Ba-PliC; PDB 4ML7; lilac), as a representative of the MliC/PliC lysozyme inhibitor family. Loop 4 is highlighted in red for rNm-ACPI and green for Ba-PliC.Images were constructed using CCP4MG [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006448#ppat.1006448.ref058" target="_blank">58</a>].