Structure-based sequence alignment of Nm-ACPI with the MliC/PliC family of lysozyme inhibitor proteins.

<p>The sequence of ACP from <i>Neisseria meningitidis</i> serogroup B strain MC58 (E6MZT7) was aligned with those for St-PliC from <i>Salmonella typhimurium</i> (Q8ZPY8); Pa-MliC from <i>Pseudomonas aeruginosa</i> (Q91574) and Ba-PliC from <i>Brucella abortus</i> (Q57ES7) based on structural superpositions carried out using CCP4MG [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006448#ppat.1006448.ref058" target="_blank">58</a>]. β-strands β1 – β8 are indicated by the shaded arrows, and are based on Nm-ACPI structure. Conserved cysteine residues of the mature proteins are blocked in black. Numbering follows the Nm-ACPI sequence. Underlined residues had weak or no density and were not included in the crystal structures. Sequence motifs involved in the interaction of <i>P</i>. <i>aeruginosa</i> MliC with Hewl are in red and green, and conserved residues within these regions are marked in bold and identified by similarly coloured asterisks (*).