Additional file 1: of MAPK-triggered chromatin reprogramming by histone deacetylase in plant innate immunity

Supplemental figures. Figure S1–S5. HD2B phosphorylation sites. Figure S6. MPK3, MPK4, MPK6, and MPK7 antibody specificity. Figure S7. MPK3, MPK4, and MPK6 substrate specificity. Figure S8. Subcellular localization of MPK3. Figure S9. BiFC analysis of HD2B interaction with MPK3, 4, and 6 in epidermal cells of Agrobacterium-infiltrated Nicotiana benthamiana. Figure S10. Co-imunoprecipitation of HD2B with MPK3. Figure S11. GO analysis of transcription factors up-regulated in hd2b mutant. Figure S12. GO analysis of downregulated genes in hd2b mutant. Figure S13. Validation of transcriptomic data by RT-qPCR. Figure S14. Susceptibility of hd2b mutant and HD2B overexpressing lines. Figure S15. Flg22-induced activation of MPK3, MPK4, and MPK6 is similar in Col-0 and hd2b mutant. Figure S16. Flg22 growth inhibition assay of hd2b mutant. Figure S17. Flagellin-induced relocalization of HD2B. Figure S18. Nuleolar HD2B and nuclear HD2B-ED localization. Figure S19. Nuclear HD2B localization. Figure S20. Gene expression analyses of hd2b and mpk3 over-expressed genes in the HD2B-AA line. Figure S21. Flagellin-independent expression of HD2B. Figure S22. Specific HD2B targets in mock conditions are involved in defense response. Figure S23. Correlation between HD2B binding and gene expression in hd2b mutants in mock and flg22 conditions. Figure S24. Characterization of H3K9 acetylated regions in mock- and flg22-treated seedlings. Figure S25. Comparison between HD2B and H3K9ac peak positions relative to TSS. Figure S26. GO analysis of H3K9-hyper and H3K9-hypo acetylated genes after flg22 treatment. Figure S27. H3K9 acetylation levels are directly correlated with expression levels after flg22 treatment. Figure S28. Gene expression analyses of hd2b and mpk3 flg22 mis-regulated genes in the HD2B-AA line. Figure S29. Characterization of hd2b mutant line. Figure S30. Positions of the regions analyzed by ChIP-qPCR experiments on the different loci. (PDF 1569 kb