Additional file 1: of H2A monoubiquitination in Arabidopsis thaliana is generally independent of LHP1 and PRC2 activity

Supplementary Tables and Figures. Table S1. Total and mapped reads produced for each ChIP-seq sample. Table S2. Primers used for ChIP qPCR. Figure S1. Comparison of H2AK121ub and H3K27me3 ChIP-seq replicates in WT Arabidopsis seedlings at 7 DAG. Figure S2. Identification of H2AK121ub peaks. Figure S3. Genome wide localization of H2AK121ub and H3K27me3 marks in Arabidopsis WT seedlings at 7 DAG. Figure S4. ChIP-qPCR validations of H2AK121ub levels in WT at 7 DAG. Figure S5. Genomic distribution of H2AK121ub marks at different categories of marked genes. Figure S6. RNA-seq analysis of WT and atbmi1a/b/c at 7 DAG. Figure S7. Different families of Transcription Factors (TFs) are enriched in H2AK121ub/H3K27me3 and only-H3K27me3 marked genes. Figure S8. Comparison of clf28/swn7 and lhp1 H2AK121ub ChIP-seq replicates at 7 DAG . Figure S9. The global levels of H2AK121ub marks are increased in clf28/swn7 mutants. Figure S10. H2AK121ub levels at H2AK121ub peaks in clf28/swn7 and lhp1 mutants compared to WT after normalization with a modified MAnorm protocol. Figure S11. ChIP-qPCR validations of H2AK121ub levels in clf28/swn7 at 7 DAG. Figure S12. Comparison of atbmi1a/b/c ChIP-seq replicates at 7 DAG . Figure S13. The global levels of H2AK121ub marks are reduced in atbmi1a/b/c mutants. Figure S14. H2AK121ub levels at selected genes in WT and atbmi1a/b/c. Figure S15. H2AK121ub levels at H2AK121ub peaks in atbmi1a/b/c mutant compared to WT after normalization with a modified MAnorm protocol. Figure S16. Genes displaying reduced levels of H2AK121ub marks were significantly enriched in genes that are transcriptionally upregulated in atbmi1a/b/c mutants. Figure S17. Loss of AtBMI1 function impacts H2AK121ub levels. Figure S18. ChIP-seq density heatmaps of H3K27me3 marks in WT and atbmi1a/b/c mutants at genomic regions surrounding the TSS of target genes. Figure S19. H3K27me3 levels at H3K27me3 peaks in atbmi1a/b/c mutant compared to WT after normalization with a modified MAnorm protocol. Figure S20. Loss of AtBMI1 function impacts H3K27me3 levels. (PDF 1398 kb