Breadth of inhibitory activity of peripheral blood CD4+CD27-CD28- Treg from CMV-seropositive donors.

<p><b>Panel A</b> shows a comparison of CMV-), VZV- and candida-specific proliferation inhibition using sorted peripheral blood CD4+CD27-CD28- Treg and autologous PBMC from 5 to 10 donors/antigen at effector to target ratios = 1:3. <b>Panel B</b> shows inhibition of proliferation of PBMC from 6 HIV-pos CMV-pos donors stimulated with CMV or HIV by autologous peripheral blood CD3+CD27-CD28- Treg at an effector to target ratio = 1:3. Inhibition of proliferation was calculated by the following formula: [(median CPM of antigen-stimulated wells) -(median CPM of antigen-stimulated + Treg wells)]/(median CPM of antigen-stimulated wells) x 100. P values above each column were calculated by single sample T test compared with 0% inhibition. P values between columns were calculated by unpaired T test. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0180691#pone.0180691.s003" target="_blank">S3 Fig</a> shows the same data presented in absolute CPM.