Functional evaluation of peripheral blood CD4+CD27-CD28- (Fresh) with <i>ex vivo</i> CMV-restimulated CD4+CD27-CD28- (Stim) T cells from CMV-seropositive donors.

<p>Bars represent means and SEM of % inhibition of proliferation calculated by the following formula: [(median CPM of CMV-stimulated wells) -(median CPM of CMV-stimulated + Treg wells)]/(median CPM of CMV-stimulated wells) x 100. E:T = Treg to autologous PBMC ratio. N = number of donors. P values above each column were calculated by single sample T test compared with 0% inhibition. P value between columns was calculated by unpaired T test. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0180691#pone.0180691.s003" target="_blank">S3 Fig</a> shows the same data presented in absolute CPM.