Lz represses <i>Notch</i> expression.

<p>(A-D) Immunostainings against NECD (Notch extracellular domain) in blood cells from <i>lz-GAL4</i>, <i>UAS-mCD8-GFP/+</i> (A), <i>lz-GAL4</i>, <i>UAS-mCD8-GFP/Y</i> (B), <i>lz-GAL4</i>, <i>UAS-mCD8-GFP/Y; UAS-lz</i> (C) and <i>lz-GAL4</i>, <i>UAS-mCD8-GFP/+; UAS-lz</i> (D) third instar larvae. NECD immunostaining only is shown in the lower panels. Nuclei were stained with Topro3. (E) Corresponding quantifications of NECD in lz>GFP⁺ blood cells. (F-F”‘) Immunostaining against Lz in circulating blood cells from <i>Notch</i>^{<i>GMR30A01</i>}<i>-GAL4</i>, <i>UAS-GFPnls</i> third instar larvae. Nuclei were stained with Topro3. (F’-F”‘): single channel images. (G-J) <i>Notch</i>^{<i>GMR30A01</i>}<i>-GAL4</i>-driven expression of GFP in circulating blood cells from larvae of the indicated genotypes. (K) Corresponding quantifications of the level of GFP. (A-D, F-J) Scale bar: 10μm. (E, K) *: p-value<0.05, *** p-value<0.001.