MLF and DnaJ-1 are required to turn down Notch expression in large crystal cells.

<p>(A) Quantificationd of the proportion of lz>GFP⁺ cells according to their size in control, <i>mlf</i>^<i>-/-</i> or <i>dnaj-1</i>^<i>-/-</i> larvae. Cells were grouped into 5 categories as compared to the mean size of lz>GFP⁺ cells in the control condition. (B-D) Quantification of NICD immunostaining (relative to control) in each of the five lz>GFP⁺ cell size categories in control (B), <i>mlf</i>^<i>-/-</i> (C) and <i>dnaj-1</i>^<i>-/-</i> (D) larvae. *:p-value<0.05, **: p-value<0.01, *** p-value<0.001, n.s.: not significant. (E-J) Fluorescent immunostainings of GFP and <i>in situ</i> hybridizations of <i>Notch</i> in circulating blood cells from <i>lz-GAL4</i>,<i>UAS-mCD8-GFP/+</i> third instar larvae of the indicated genotypes. Representative images of <i>Notch</i> expression in small/medium (E, G, I) <i>versus</i> large (F, H, J) lz>GFP⁺ cells. Scale bar: 10 μm. Nuclei were stained with Topro3. The lower panels show <i>Notch</i> expression only.