Enhanced resistance of the <i>wrky54wrky70</i> double mutant to the necrotrophic fungus <i>Botrytis cinerea</i> strain B05.10.

<p>(A) Three-week-old Arabidopsis plants were inoculated with <i>B</i>. <i>cinerea</i> strain B05.10 (pipetting the spore suspension to three leaves of each plant). Seventy-two plants of each line in one tray were used in one independent experiment. The representative plants were photographed 48h after inoculation. Three independent experiments were performed with similar results. (B) The disease symptom was evaluated by measuring the average lesion diameters on three leaves of eight plants each. Values were mean ± SD of three independent experiments (*, P<0.01, one-way ANOVA test). (C) Three spraying-inoculated leaves of five plants each from three-week-old plants were stained with trypan blue 48h after infection to visualize the cell death. Three independent experiments were performed with similar results.