Identification of divergent gene expression patterns between B2 and B19 macrophages.

<p>Visualization of divergent gene expression patterns between the B2 and B19 haplotypes. A subset of genes exhibiting divergent gene expression were identified and visualized in heat following hierarchical clustering of the genes (rows), but not the time points (columns). The genes cluster into four major clades (clade1, clade2, clade3, and clade4) with a singleton gene (labelled clade 5). Among these genes, represented in clade1 and clade2, are a number of miRNAs exhibiting strong expression in B2 cells (mir-147, mir-146b, mir-1618, mir-200a, mir-1649, and mir-1648a) compared to the B19 samples. Likewise, miRNAs contained in clade3 and clade4 exhibit greater expression in B19 cells (mir-1627, mir-222b, mir-1633, and mir-19a). Additionally, a number of small nucleolar RNAs (snoRNAs) exhibit similarly dichotomous gene expression patterns (clade4) such that SNORd24, snoZ40, SNORD74, SNORA17, and SNORD12 exhibit substantially higher levels of expression in B19 cells on day -6 compared to B2 cells while B2 cells express such as snoU2_19 (clade2).