Immunohistochemical analysis of total FAK and pFAK-Y397 expression in osteosarcoma tissues.

<p>(A, B) Negative expression, (C, D) weak expression, (E, F) moderate expression, (G, H) strong expression. (I, J) Strongly diffused total FAK (I-right) or pFAK-Y397 (J-right) expression in osteosarcoma, in contrast to diminished total FAK (I-left) or pFAK-Y397 (J-left) expression in adjacent epiphyseal growth plates. Original magnifications, x400 (A-H), x100 (I, J). Analysis of immunohistochemistry of total FAK and pFAK-Y397. (K) Percentage of positive tumor cells classified into 4 categories: 0, no staining; 1, < 25%; 2, 25–49%; 3, 50–74%; 4, 75–100%. (L) Staining intensity of positive tumor cells: 0, none; 1, weak; 2, moderate; 3, strong. (M) Overall score for total FAK or pFAK-Y397 expression in patients with osteosarcoma. The scores from staining percentage and intensity were added to yield the overall scores of 0, negative; 1–2, weak; 3–4, moderate; 5–7, overexpression.