Immunohistochemical analysis of tumors with adipogenic or osteogenic origin.

<p>A: A biopsy from a liposarcoma with an adipogenic and a myogenic metastasis was investigated for expression of Dlk1, Desmin, Myogenin, Pax7 and NCAM. All myogenic markers were predominantly detected in the myogenic metastasis and not in the main liposarcoma or the adipogenic metastasis. Accordingly, Dlk1 was primarily expressed in the myogenic metastasis. Few Dlk1 positive cells could, however, be detected in the adipogenic metastasis, where few cells were found to express Desmin and Myogenin as well. This supports the observation that Dlk1 is a strong marker for tumors with skeletal muscle phenotype. Scalebar representative for all images: 50 µm. B: Biopsies from tumors with osteogenic origin were analyzed for expression of Dlk1 and all types analyzed (osteosarcoma, osteochondroma and osteoblastoma), were negative for Dlk1 protein expression. Scalebar representative for all images: 50 µm.