Histological and immunohistochemial analysis of rat’s liver.

<p>(A): H&E staining of rat’s liver after 4 weeks of cell sheet transplantation. (a) Normal rat liver cells. (b) Morphology of liver after cancer cells transplantation, black arrow shows hepatic cancer, whereas green arrow shows normal hepatocytes. Blue arrow shows inflamed cell and orange arrow represent necrotic HCC cells, x20 magnification. (B): IHC staining of tumour marker GPC3 antibody on rat’s liver after 4 weeks of transplantation. (a) Negative control, normal rat, no transplantation. (b) HepG2 cell sheet, (c) BM-SC and HepG2 cell sheet on the rat with tumour formation, (d) UC-SC and HepG2 cell sheet on the rat with tumour formation. (e) UC-SC and HepG2 cell sheet on the rat without tumour formation. x10 magnification.