Additional file 4: Figure S3. of Sonic hedgehog (SHH) signaling improves the angiogenic potential of Wharton’s jelly-derived mesenchymal stem cells (WJ-MSC)

WJ-MSC and AD-MSC respond differentially to SHH pathway stimulation. Cells were treated with Pur or N-Shh, and the response was determined by measuring the levels of (A) PTCH1, (B) GLI1, and (C) ANGPT1 by qPCR. WJ-MSC proved to be more responsive to SHH pathway stimulation than AD-MSC. (WJ-MSC n = 4; AD-MSC n = 5; *P < 0.05 unpaired Student’s t test for comparison between AD and WJ-MSC in each treatment). (D) Quantification of basal levels of PTCH1, GLI1, and ANGPT1 of WJ-MSC when compared to AD-MSC. Expression levels of the three genes were lower in WJ-MSC cultures (WJ-MSC n = 4; AD-MSC n = 5). (TIF 1095 kb