The effect of HuR expression levels on viability of SW620 cells.

<p><b>(A) The knockdown efficiency of siHuR and its effect on cell viability.</b> SW620 cells were transfected with 75 nM of scramble siRNA (siC) or 0, 25, 50, 75 nM of siRNA against HuR (siHuR) for 48 h. Cells without transfection are designed as control (CTR). Proteins were then extracted for detection of HuR and Calnexin by western blotting (upper panel) and the quantification of relative HuR/Calnexin expressions was shown. Data are presented as means ± S.D. from three independent experiments (lower left panel). * P<0.05 compared to the control group; ^# P<0.05 compared to the siC group; ^Φ P<0.05 compared to siHuR 25 nM group. The effect of siHuR on the viability of SW620 cells were measured at 0, 25, 50, 75 nM of siHuR for 24 h. Viable cells were measured by MTT assay. The percentage of cell viability was calculated by setting the control group as 100%. Data are presented as means ± S.D. from three independent experiments (lower right panel). * P<0.05 compared to the control group; ^# P<0.05 compared to the siC group. <b>(B) The overexpression efficiency of HuR and its effect on cell viability.</b> Cells were transfected with pCDNA-HA (HA) 4 μg or 0, 1, 2, 3, 4 μg/ml of pCDNA-HA-HuR (HuR) for 48 h. Cells without transfection were designed as control. Proteins were then extracted for detection of HuR and Calnexin by western blotting (upper panel). The effect of HuR on the cell viability was measured at 0, 1, 2, 3, 4 μg/ml of HuR for 24 h. The viable cells were measured and analyzed as in (A). The plot was shown in the lower panel of (B). * P<0.05 compared to the control group; ^# P<0.05 compared to the HA group.