Stronger chlamydicidal activities in SF1, SF2 and SF3, as compared to CF0002.

<p>HeLa cells were infected with RFP/iGFP-L2r at an MOI of 0.2 inclusion-forming unit per cell. Chemical treatment started 1 h postinoculation. 40 h postinoculation, culture media were removed; cell lysates were prepared, subjected to 1:10 serial dilution and inoculated onto L929 monolayers. Infected L929 cells were cultured in the presence of anhydrotetracycline to induce GFP expression. GFP-expressing inclusions were enumerated 30 h later. Data are averages ± standard deviations of duplicate experiments. Single and double asterisk denote statistically significant differences between N-acylhydrazone-treated cultures and control DMSO-treated cultures (P < 0.05 and P < 0.01, respectively, two-tailed Student’s t test).