Growth of <i>gdh1</i>Δ, <i>gdh2</i>Δ, <i>gln1</i>Δ, and <i>glt1</i>Δ mutants on various nitrogen sources.

<p>Five-microliter aliquots of cell suspension (5 x 10⁴ cells) were spotted onto glucose minimal medium containing 10 mM ammonium (NH₄⁺), 10 mM glutamate (Glu), or 10 mM glutamine (Gln) (A), or 0.1, 1, 10, or 100 mM ammonium (B) as the nitrogen source. The plates were incubated at 30°C for 2 days before photographs were taken. Strains are the same set of strains as used for enzyme assays: HMP126 (WT), HMP135 (<i>gdh1</i>Δ), HMP128 (<i>gdh2</i>Δ), HMP141 (<i>gln1</i>Δ), and HMP125 (<i>glt1</i>Δ).