GlnPQ is required for L-glutamine utilization by <i>L</i>. <i>monocytogenes</i>.

<p>(A) Growth of WT <i>L</i>. <i>monocytogenes</i> (WT circles) or Δ<i>glnPQ</i> (squares) in MDM supplemented with 1 mM L-glutamine as the sole nitrogen source. Shown are representative growth curves of 3 independent experiments, performed in triplicates. (B) Optical density (600 nm) measurements after 30 h of growth of WT <i>L</i>. <i>monocytogenes</i> (circles), Δ<i>glnPQ</i> (squares), Δ<i>glnPQ</i>-pGlnPQ (diamonds), or <i>glnQ</i>-E164A (triangles) bacteria in MDM supplemented with the indicated concentrations of L-glutamine as the sole nitrogen source. Results are mean ±SD of 3 independent experiments, performed in triplicates.