Surface MR expression and effect of its blockade on DENV replication in MDMs and D₃-MDMs.

<p>MR surface expression was measured by FACS detection of CD206+ cells after MDM and D₃-MDM differentiation. <b>A.</b> Flow cytometry-gating strategy for the measurement of CD206+ events from the parental region A from <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0005904#pntd.0005904.s001" target="_blank">S1B Fig</a>. Isotype controls were used for both MDMs and D₃-MDMs to set the CD206 positive events gate. Middle and lower panels show representative distribution of CD206+ cells in MDM and D₃-MDM in gate B and the comparison of CD206 Mean Fluorescence Intensity (MFI). <b>B</b>. Statistical comparison of CD206+ percentage cells and CD206 MFI. <b>C.</b> Correlation between the percentage of CD206 positive cells and infection percentage in MDMs and D₃-MDMs observed in 4 different donors. <b>D.</b> MR ligation to DENV-2 was blocked by incubating with methyl mannoside (MM) for 2 h prior to infection. The intracellular number of GEc was measured by RT-qPCR 24 hpi and was compared with that in control mock-treated cells. Bars represent mean ± SD from at least 3 different donors. Wilcoxon signed rank test; *<i>p</i><0.05; n.s denotes non-significant.