Expression of activation markers on Ly6C^high monocytes, moDCs, cDCs and MΦs.

<p><i>Ifnar</i>^–/– mice were inoculated i.d. with DENV2 during 1° or ADE infection. (A and B) Bar graphs show mean ±SEM of CD80 (B7-1) (A) or CD86 (B7-2) (B) surface protein expression determined via flow cytometric analysis and calculated as median fluorescence intensities (MFI) of specific stains divided by the MFI of isotype-matched control stains. CD80 and CD86 expression was examined on Ly6C^high monocytes, Ly6C⁺ CD11b⁺ moDCs, Ly6C^– CD11b⁺ cDCs, and MΦs during steady state, or 48 hpi with DENV under 1° or ADE conditions. In DENV-inoculated samples, each cell population was gated further on the presence of intracellular DENV proteins NS3 and E for DENV-infected cells (DENV⁺) or the absence of DENV proteins for non-infected bystander cells (DENV^–). Significant differences in activation marker expression after i.d. inoculation with DENV compared to steady state are marked as * for <i>p</i>≤0.05 and ** for <i>p</i>≤0.01 above individual bars. Significant differences between DENV^– and DENV⁺ within the same sample are marked between bars with brackets and were calculated using the paired <i>t</i>-test. Pooled data from two independent experiments (<i>n</i> = 6 per condition).