Additional file 2: Figure S2. of Sequence analysis and characterization of pyruvate kinase from Clonorchis sinensis, a 53.1-kDa homopentamer, implicated immune protective efficacy against clonorchiasis

Apparent Mr. of rCsPK. a Determination of the Mr. of rCsPK using 8% native PAGE according to Fig. 3b. After plotting the HRm values of the standard markers against their Mrs., we drew a curve, fitted an equation to the curve, and used the equation for calculating the Mr. of rCsPK. b Elution profile of rCsPK in GFC. c Mr. of rCsPK as detected with GFC. The calibration curve relating the elution volumes (Ve) and the log Mr. (kDa) of standard marker proteins were obtained with AKTA FPLC using a Sepharose 12 10/300 GL GFC column. Based on the deduced equation, the Mr. of rCsPK was calculated from the obtained Ve of rCsPK. (TIFF 252 kb