<p><i>In vivo stimulus</i>: insulin 2IU/Kg (b.w) (i.v.). Graphs present mean O.D. ± S.E.M of the bands and n represents the number of animals used in each group. Studied groups: Control (n = 3); HFD (n = 3) and GK (n = 3). (*) p<0.05 <i>vs</i> control (+); (**) p<0.01 <i>vs</i> control (+); ($ $ $) p<0.001 as indicated in the graphs.</p
<p>Differentiated human subcutaneous SGBS adipocytes <b>(A)</b> and visceral LiSa-2 adipocytes <b>(B...
<p>(A) Reduction of basal striatal Akt activity in HF- vs. LF-fed animals was determined by phosphor...
<p>Crude hippocampal extracts (5–15 μg/lane) were analyzed by Western blots developed with antibodie...
<p>Positive stimulus: insulin (7 nM). Graphs present mean O.D. ± S.E.M of the bands and n represents...
<p><b>Panel A:</b> Western blot analysis of P-Akt/Akt <i>ratio</i> in SAT with and without incubatio...
<p>For quantification, each blot has been hybridized to α-tubulin and the graphs below represent mea...
EDL, extensor digitorum longus; WAT, white adipose tissue. Bar graphs represent the ratio of the val...
<p>WT (black bars) and 12/15LO KO (gray bars) mice were fed HFD for two weeks. Mice were then inject...
Levels of p-AKT (Ser473) and total AKT were measured using a plate assay in whole brain protein extr...
<p>A-C: Western Blot analysis in brain tissues after intravenous (i.v.) or intracerebroventricular (...
<p>A and B, Animals were stimulated with 5 units of human insulin (saline as a vehicle control) for ...
<p><i>A</i>: Subcutaneous white adipose tissue immunoblots of insulin signaling components in chow- ...
<p>(<b>A)</b> Representative western blot analysis of the expression and activation of proteins invo...
<p>The figure shows a Western blot of a representative experiment (top) and densitometric analyses f...
pAKT abundance was determined in NV and E4 cells treated with no insulin (Control, A), insulin (B) o...
<p>Differentiated human subcutaneous SGBS adipocytes <b>(A)</b> and visceral LiSa-2 adipocytes <b>(B...
<p>(A) Reduction of basal striatal Akt activity in HF- vs. LF-fed animals was determined by phosphor...
<p>Crude hippocampal extracts (5–15 μg/lane) were analyzed by Western blots developed with antibodie...
<p>Positive stimulus: insulin (7 nM). Graphs present mean O.D. ± S.E.M of the bands and n represents...
<p><b>Panel A:</b> Western blot analysis of P-Akt/Akt <i>ratio</i> in SAT with and without incubatio...
<p>For quantification, each blot has been hybridized to α-tubulin and the graphs below represent mea...
EDL, extensor digitorum longus; WAT, white adipose tissue. Bar graphs represent the ratio of the val...
<p>WT (black bars) and 12/15LO KO (gray bars) mice were fed HFD for two weeks. Mice were then inject...
Levels of p-AKT (Ser473) and total AKT were measured using a plate assay in whole brain protein extr...
<p>A-C: Western Blot analysis in brain tissues after intravenous (i.v.) or intracerebroventricular (...
<p>A and B, Animals were stimulated with 5 units of human insulin (saline as a vehicle control) for ...
<p><i>A</i>: Subcutaneous white adipose tissue immunoblots of insulin signaling components in chow- ...
<p>(<b>A)</b> Representative western blot analysis of the expression and activation of proteins invo...
<p>The figure shows a Western blot of a representative experiment (top) and densitometric analyses f...
pAKT abundance was determined in NV and E4 cells treated with no insulin (Control, A), insulin (B) o...
<p>Differentiated human subcutaneous SGBS adipocytes <b>(A)</b> and visceral LiSa-2 adipocytes <b>(B...
<p>(A) Reduction of basal striatal Akt activity in HF- vs. LF-fed animals was determined by phosphor...
<p>Crude hippocampal extracts (5–15 μg/lane) were analyzed by Western blots developed with antibodie...