Western blot analysis of insulin signal transduction in rat hippocampus.

<p>Crude hippocampal extracts (5–15 μg/lane) were analyzed by Western blots developed with antibodies to total AKT and phosph-AKT (4A, B), total GSK3β and phosph-GSK3β (4C, D). Actin blot was included as a loading control. Each lane was from an individual rat (4A, C), the blots as shown in panel 4B, D were quantitated desitometrically and for quantitation of total AKT and total GSK-3β. All data are presented as mean ± SD of the relative immunoreactivities. *<i>P < 0</i>.<i>05</i>,**<i>P < 0</i>.<i>01</i> as compared to NC group; ⊿<i>P < 0</i>.<i>05</i> as compared to T2D group and #<i>P < 0</i>.<i>05</i> as compared to T2D+PF group.