Additional file 1: of Isolation and characterization of HepP: a virulence-related Pseudomonas aeruginosa heparinase

Figure S1. Characteristics of HepP and its homology to other proteins. Figure S2. Amino acid sequence of the 78-kDa predicted protein encoded by zbdP. Figure S3. Nucleotide sequences of zbdP and hepP showing locations of primers used in experiments to analyze these two genes. Figure S4. Mutation of hepP or zbdP does not significantly alter the growth of PA14 in vitro. Figure S5. Detailed experiment to confirm transcription of hepP in PA14ΔzbdP. Table S1. Primers used to compare virulence factor gene transcription in PA14, PA14ΔhepP and PA14ΔzbdP. (PDF 1972 kb