Additional file 2: Figure S2. of Nc886 is epigenetically repressed in prostate cancer and acts as a tumor suppressor through the inhibition of cell growth

Hierarchical cluster of 50 paired normal and tumor tissue of PrCa patients from TCGA-PRAD dataset. A. 50 normal prostate tissue B. 50 prostate tumor tissues. Clusterization was performed by Gene-E Euclidean algorithm using default parameters. The two major clusters, indicated as “high” and “medium” methylation, were selected for further clinical association studies shown in D and F. The average methylation of 10 CpG sites of nc886 promoter (including the 6 sites of the TSS200, 1 located at the gene body and 3 located 200-350pb upstream of the TSS200) is shown for normal (C) and tumor tissue (E). The clinical variables reported at the TCGA-PRAD were analyzed for correlations with the methylation status of nc886 promoter, and only the statistically significant associations found are shown. D. Average clinical T value is associated with the methylation status of the normal tissue (normal) of the patient. F. Average pathological T value is associated with the methylation status of the tumor tissue (tumor) of the patient. *P-value < 0.05; ** P-value < 0.01; *** P-value < 0.001 two-tailed t-test. (PDF 419 kb