Additional file 5: Figure S2. of DNA hypermethylation and decreased mRNA expression of MAL, PRIMA1, PTGDR and SFRP1 in colorectal adenoma and cancer

Box plot representation of DNA methylation alterations of analysed genes in biopsies and macrodissected specimens of NAT (n = 10), AD (n = 10), and CRC (n = 10) and in LCM epithelial cells of NAT (n = 5), AD (n = 5) and CRC (n = 5). Red dots are individual DNA methylation percent values, box plots represent median and standard deviation of the data. Asterisk (*) represents significance (p < 0.05) in adenoma and tumour samples compared to normals and double asterisk (**) represents significance (p < 0.05) in tumour samples compared to adenomas. Each box plot has individual scale of DNA methylation percent. Certain genes showe hypermethylation in colon biopsies and also in LCM epithelial cells (e.g. MAL, PRIMA1, PTGDR, SFRP2), while there were genes with no remarkable DNA methylation level alterations (e.g. BCL2, CDX1, PTGS2, SULF1). Macro = macrodissected samples, LCM = laser microdissected epithelial cells. (PDF 359 kb