Added functionality: Deduplicating on gene ids ( #44 for motivation): The user can now group/dedup according to the gene which the read aligns to. This is useful for single cell RNA-Seq methods such as e.g CEL-Seq where the position of the read on a transcript may be different for reads generated from the same initial molecule. The following options may be used define the gene_id for each read: --per-gene --gene-transcript-map --gene-tag Working with BAM tags (#73, #76, #89): UMIs can now be extracted from the BAM tags and . See following options for controlling this behaviour: --extract-umi-method --umi-tag --umi-group-tag Ouput unmapped reads (#78) The group command will now output unmapped reads if the --output-unmapped is supplied. T...
Motivation: Next-generation sequencing has become an important tool in molecular biology. Various pr...
Unique Molecular Identifiers (UMIs) are random oligonucleotide barcodes that are increasingly used i...
<p>Numbers of reads in RPM (reads per million mapped reads) are shown on the Y-axis and the genomic ...
Added functionality: Deduplicating on gene ids ( #44 for motivation): The user can now group/dedup ...
Added functionality: Deduplicating on gene ids ( #44 for motivation): The user can now group/dedup ...
Tweaks the way group handles paired end BAMs. To simplify the process and ensure all reads are writt...
Version 0.5.0 introduces new commands to support single-cell RNA-Seq and reduces run-time. The under...
New features Adds '--umi-separator' option to umi_tools extract to specify UMI separator. Thanks @o...
Adds the new group command to group PCR duplicates and return the groups in a tagged BAM file and/or...
Minor update. Improves detection of duplicate reads with paired end reads, reduces run time with ded...
A long overdue release covering some minor functionality updates and bugfixes: Additional functional...
When investigating differences in expression matrixes generated by 10x and our software, we discover...
Improves the group command: Adds the --subset option as per the dedup command (#74) Corrects the fl...
Data analyzed in Lause, Ziegenhain et al. (2023). Code to obtain these tables from public data sour...
The Barcode-UMI-Set format (BUS) is a recently developed format for representing pseudoalignments of...
Motivation: Next-generation sequencing has become an important tool in molecular biology. Various pr...
Unique Molecular Identifiers (UMIs) are random oligonucleotide barcodes that are increasingly used i...
<p>Numbers of reads in RPM (reads per million mapped reads) are shown on the Y-axis and the genomic ...
Added functionality: Deduplicating on gene ids ( #44 for motivation): The user can now group/dedup ...
Added functionality: Deduplicating on gene ids ( #44 for motivation): The user can now group/dedup ...
Tweaks the way group handles paired end BAMs. To simplify the process and ensure all reads are writt...
Version 0.5.0 introduces new commands to support single-cell RNA-Seq and reduces run-time. The under...
New features Adds '--umi-separator' option to umi_tools extract to specify UMI separator. Thanks @o...
Adds the new group command to group PCR duplicates and return the groups in a tagged BAM file and/or...
Minor update. Improves detection of duplicate reads with paired end reads, reduces run time with ded...
A long overdue release covering some minor functionality updates and bugfixes: Additional functional...
When investigating differences in expression matrixes generated by 10x and our software, we discover...
Improves the group command: Adds the --subset option as per the dedup command (#74) Corrects the fl...
Data analyzed in Lause, Ziegenhain et al. (2023). Code to obtain these tables from public data sour...
The Barcode-UMI-Set format (BUS) is a recently developed format for representing pseudoalignments of...
Motivation: Next-generation sequencing has become an important tool in molecular biology. Various pr...
Unique Molecular Identifiers (UMIs) are random oligonucleotide barcodes that are increasingly used i...
<p>Numbers of reads in RPM (reads per million mapped reads) are shown on the Y-axis and the genomic ...