Add to ORKGRecording the activity of large populations of neurons is an important step toward understanding the\ua0emergent function of neural circuits. Here we present a simple holographic method to simultaneously perform two-photon calcium imaging of neuronal populations across multiple areas and layers of mouse cortex in\ua0vivo. We use prior knowledge of neuronal locations, activity sparsity, and a constrained nonnegative matrix factorization algorithm to extract signals from neurons imaged simultaneously and located in different focal planes or fields of view. Our laser multiplexing approach is simple and fast, and could be used as a general method to image the activity of neural circuits in three dimensions across multiple areas in the brain.R01...
Recent years have witnessed major improvements in our understanding of the brain, thanks to technolo...
Summary: Sensory information is encoded within the brain in distributed spatiotemporal patterns of n...
Optical control of neural ensemble activity is crucial for understanding brain function and disease,...
Recording the activity of large populations of neurons is an important step toward understanding the...
Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neuron...
The simultaneous imaging and manipulating of neural activity could enable the functional dissection ...
Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neuron...
Two-photon excitation fluorescence microscopy is widely used to study the activity of neuronal circu...
To capture the emergent properties of neural circuits, high-speed volumetric imaging of neural activ...
Spontaneous and sensory-evoked activity propagates across varying spatial scales in the mammalian co...
In vivo 2-photon calcium imaging would benefit from the use of multiple excitation beams to increase...
In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increa...
Information exchange between neural systems occurs at the level of populations of neurons. Thus in o...
High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge ...
Current in vivo methods for imaging the visual cortex lack the ability to map response properties at...
Recent years have witnessed major improvements in our understanding of the brain, thanks to technolo...
Summary: Sensory information is encoded within the brain in distributed spatiotemporal patterns of n...
Optical control of neural ensemble activity is crucial for understanding brain function and disease,...
Recording the activity of large populations of neurons is an important step toward understanding the...
Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neuron...
The simultaneous imaging and manipulating of neural activity could enable the functional dissection ...
Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neuron...
Two-photon excitation fluorescence microscopy is widely used to study the activity of neuronal circu...
To capture the emergent properties of neural circuits, high-speed volumetric imaging of neural activ...
Spontaneous and sensory-evoked activity propagates across varying spatial scales in the mammalian co...
In vivo 2-photon calcium imaging would benefit from the use of multiple excitation beams to increase...
In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increa...
Information exchange between neural systems occurs at the level of populations of neurons. Thus in o...
High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge ...
Current in vivo methods for imaging the visual cortex lack the ability to map response properties at...
Recent years have witnessed major improvements in our understanding of the brain, thanks to technolo...
Summary: Sensory information is encoded within the brain in distributed spatiotemporal patterns of n...
Optical control of neural ensemble activity is crucial for understanding brain function and disease,...