Studies on the Reduction Process of Bilirubin in the Intestine Part 1 Studies on the separation of bilirubinoide in feces

Bilirubinoide in the feces of various patients was separated by column chromatography. And the results were as follows. 1. Bilirubin and urobilin could be separated from the feces of various patients, but other bilirubinoide was only identified by the absorption curves because of the scanty dosis. 2. Bilirubin, mesobilirubin, dihydromesobilirubin and urobilin were separated from the feces of 6 cases in the 8 cases with the administration of antibiotics for a long period, and bilirubin, dihydrobilirubin, mesobilirubin and urobilinogen in other one case and bilirubin, dihydromesobilirubin in another case. 3. In the above 2 cases with the administration of antibiotics, mesobilirhodin and mesobiliviolin were identified in the mixture of urobilin, but each fraction of them could be scarcely separated. Since dihydromesobilirubin was separated at that time, it was thought that mesobilirhodin and mesobiliviolin were produced by the oxydation of dihydromesoblilirubin during the separation process. Therefore, it was thought that dihydromesobilirubin produced in the intestine was composed of two isomerides. 4. The formation of urobilinogen in the intestine was supposed to take the same reduction process of bilirubin by colloidal palladium in vitro