with lectin-like oxidized LDL receptor-1 and mediates oxidized LDL-in-

datively modified low-density lipoprotein (OxLDL) is a contributing factor of endothelial dysfunction, an early cellular event during atherogenesis. In endothelial cells, OxLDL has been shown to stim-ulate proinflammatory responses, increase lipid accumulation, and induce the expression of adhesion and extracellular matrix degrading molecules. The primary receptor for OxLDL on endothelial cells has been identified as a member of the scavenger receptor family called lectin-like OxLDL receptor-1 (LOX-1). A number of studies on LOX-1 have implicated its role in multiple cardiovascular diseases including atherosclerosis. To better understand the molecular mech-anisms underlying the role of LOX-1 in endothelial cells, we identi-fied interacting proteins in an affinity-purified LOX-1 receptor com-plex from human aortic endothelial HAECT cells by mass spectrom-etry. Two molecules involved in Rho signaling pathway, ARHGEF1 and ROCK2, were identified, and their associations with LOX-1 wer