On-Line FTIR Detection in Small-Bore Liquid Chromatography

The development of on-line Fourier transform infrared (FTIR) detect ion of normal-phase, smal l-bore (1.0-mm × 50-cm), h igh per formance l iqu id chromatograph ic e f f luents is re v iewed. Sensi t iv i ty and mobi le phase cons idera t ions are d iscussed. The appl icabi l i ty of the technique t o nonpolar, polar, and very polar components in complex coal-derived mixtures is described. Limitat ions of the method along wi th ant ic ipated fu ture deve lopments are o u t l i n e d. Introduct ion The analysis of highly complex mixtures places severe de mands upon analytical methodology. These demands vary depending upon the nature o f the complex mixture. For exam ple, the chemical classes represented in the mixture, the matrix wherein the mixture is contained, and the relative concentratio