Enzyme Histochemistry and Immunohistochemistry on Biopsy Specimens of

We have systematically investigated a variety of fixation and plastic embedding procedures and arrived at a method that allows processing of-2-zm sections of bone marrow biopsies for examination by light microscopy. More impor-tantly, this method permits the use of enzyme histochemi-cal and immunohistochemical procedures that are rapidly becoming mandatory in the diagnosis of hematologic malignancies. Over 200 full-length bone marrow biopsy specimens were fixed in a mixture of paraformaldehyde. glutaraldehyde, and acrolein. dehydrated in acetone, and embedded in a mixture of methyl and glycolmethacrylate. All procedures were carried out at 4#{176}C.Decalcification was unnecessary. Sections 2-Mm thick were cut and incubated T HE PARAFFIN-EMBEDDED bone marrow biopsy specimen is very valuable in the evalua