Evaluation of the Performances of Ribosomal Database Project (RDP) Classifier for Taxonomic Assignment of 16S rRNA Metabarcoding Sequences Generated from Illumina-Solexa NGS

Published: 2015.02.01 Here we report a benchmark of the effect of bootstrap cut-off values of the RDP Classifier tool in terms of data retention along the different taxonomic ranks by using Illumina reads. Results pro-vide guidelines for planning sequencing depths and selection of bootstrap cut-off in taxonomic assignments. Key words: 16S rRNA; metabarcoding; ribosomal database project; OTU clustering; bacterial communities