Classifiability of 16S sequence data is differentially impacted by sequencing technology, taxonomic family and body region.

<p>For each of the HMP body regions, the relationship between the average frequency of a given bacterial family (y-axis) versus the contribution of these families to the unclassifiability issue (x-axis) is plotted for (B) 3730 and (C) 454. Only window V3–V5 is presented in 454 results. Classification was performed on quality- and chimera-filtered sequences and classifications assigned only if the RDP Classifier result had a confidence score above 80%.