Add to ORKGIntroduction—Bisulfite treatment of DNA introduces methylation-dependent sequence changes through selective chemical conversion of nonmethylated cytosine to uracil and serves as pretreatment step for the majority of DNA methylation analysis methods. Methods—We have evaluated the conversion performance of five of the most commonly used bisulfite treatment kits [MethylDetector (Active Motif), Epitect+ (Qiagen), Zymo Methylation
Bisulfite (BS) conversion, which includes a series of chemical reactions using bisulfite, is a prere...
<p>To analyze the allele-specific DNA methylation, we individually measured the DNA methylation of e...
<p>DNA was bisulfite converted using nine different commercially available kits. Each kit was proces...
IntroductionBisulfite treatment of DNA introduces methylation-dependent sequence changes through sel...
DNA methylation is the most studied epigenetic modification with a wide range of regulatory function...
DNA methylation is an essential epigenetic modification in the human genome. For the investigation o...
Bisulfite genomic sequencing is a widely used technique for analyzing cytosine-methylation of DNA. B...
Epigenetic alterations, including DNA methylation, play an important role in the regulation of gene ...
Epigenetic alterations, including DNA methylation, play an important role in the regulation of gene ...
The last few years have seen a growing interest in the study of DNA methylation because of its now a...
After bisulfite treatment, (A) the internal control (IC) used to monitor bisulfite conversion effici...
Methylation, the addition of methyl groups to cytosine (C), plays an important role in the regulatio...
DNA methylation analyses usually require a preceding bisulfite conversion of the DNA. The choice of ...
<p>5-mc: 5-methylcytosine; 5-hmc: 5-hydroxymethylcytosine; 5-ms: 5-methylenesulfonate; 5-hmu: 5-hydr...
DNA methylation is one of the most important epigenetic modifications in the regulation of gene tran...
Bisulfite (BS) conversion, which includes a series of chemical reactions using bisulfite, is a prere...
<p>To analyze the allele-specific DNA methylation, we individually measured the DNA methylation of e...
<p>DNA was bisulfite converted using nine different commercially available kits. Each kit was proces...
IntroductionBisulfite treatment of DNA introduces methylation-dependent sequence changes through sel...
DNA methylation is the most studied epigenetic modification with a wide range of regulatory function...
DNA methylation is an essential epigenetic modification in the human genome. For the investigation o...
Bisulfite genomic sequencing is a widely used technique for analyzing cytosine-methylation of DNA. B...
Epigenetic alterations, including DNA methylation, play an important role in the regulation of gene ...
Epigenetic alterations, including DNA methylation, play an important role in the regulation of gene ...
The last few years have seen a growing interest in the study of DNA methylation because of its now a...
After bisulfite treatment, (A) the internal control (IC) used to monitor bisulfite conversion effici...
Methylation, the addition of methyl groups to cytosine (C), plays an important role in the regulatio...
DNA methylation analyses usually require a preceding bisulfite conversion of the DNA. The choice of ...
<p>5-mc: 5-methylcytosine; 5-hmc: 5-hydroxymethylcytosine; 5-ms: 5-methylenesulfonate; 5-hmu: 5-hydr...
DNA methylation is one of the most important epigenetic modifications in the regulation of gene tran...
Bisulfite (BS) conversion, which includes a series of chemical reactions using bisulfite, is a prere...
<p>To analyze the allele-specific DNA methylation, we individually measured the DNA methylation of e...
<p>DNA was bisulfite converted using nine different commercially available kits. Each kit was proces...