Published By: Blue Eyes Intelligence Engineering & Sciences Publication Pvt. Ltd. Differentiation of Oral Streptococcal Species by Haemolysis in Blood Agar Medium in Vitro

Abstract- Pathogenic bacteria also cause infections such as tetanus, typhoid fever, diphtheria, syphilis, and leprosy. Blood agar medium contain a typical nutrient growth medium enriched with 5 % sheep blood. It is useful for encouraging growth of fastidious organisms. The Three Types of Lytic Activities seen on the plate are- clear zone around bacterial growth-RBC hemolyzed completely (Beta-hemolysis, pathogenic); green zone around growth-RBC partially hemolyzed (Alpha-hemolysis); no change around growth-RBC is not hemolyzed (Gamma-hemolysis or no hemolysis). The discrimination of oral bacteria specifically Streptococcal species can be done by haemolysis tests using blood agar medium. Other than ultramicroscopic and morphological studies this experiment also conveys the species differentiation due to its lytic activity. Thus, blood agar medium can be a useful medium for pathogenic bacterial survey and investigation. The present review has proved the importance of blood agar utilization in diagnostics and disease analysis