Berlin · New York Influence of 2(3-Methyl-cinnamyl-hydrazono)-propionate on Glucose

Summary: 2-(3-Methyl-cinnamyl-hydrazono)-propionate stimulated glucose oxidation in human mono-nuclear leukocytes and the Stimulation was similar to that by concanavalin A. Both substances must affect glucose metabolism at two sites, the first site being before the pyruvate dehydrogenase Step because of the increase of lactate plus pyruvate concentration. The second site is related to pyruvate oxidation. The hydrazone inhibited the conversion of palmitate to CO2. This effect could have caused an activation of the pyruvate dehydrogenase complex, resulting from a decreased acetyl-CoA/CoA ratio. Concanavalin A did not influence fatty acid oxidation. Both substances did not affect the CO2 forrnation from acetate. Mononuclear leukocytes appear to be a suitable model f r the investigation of the influence of hypoglycaemic substances on glucose and fatty acid metabolism in living human cells. Introduction Human mononuclear blood cells have become in-„ j · ·- j i _ i _ r · j i creasingly useful f r the study of insulin receptorsHydrazonopropiomc acids have been found to lower, _ ' ,.,-.-. „ *u u-i Λ i ~ ' ' Γ ' V... ' , ' (7—10) and vanous metabohc pathways, especmllythe blood glucose concentration in vanous laboratory:., ". ' ,,.,44 * —, ON _ /0 ~f, t., « j \ m glucose and fatty acid metabolism (11 — 13). Theammals (1—3). 2-(3-Methyl-cmnamyl-hydrazono)-. _ ,