and Glucose Oxidation in the Isolated Rat Diaphragm Using 14C-Labelled Substrates Hydrazonopropionic Acids, a New Class of Hypoglycaemic Substances, VIII1

Summary: The influence of 2-(3-methyl-cinnamyl-hydrazono)-propionate on the utilization of various sub-strates in isolated rat hemidiaphragms was investigated in comparison with other hypoglycaemic compounds. The effect of 2-(3-methyl-cinnamyl-hydrazono)-propionate was concentration-dependent. At a concentration of 0.5 mmol/1 2-(3-methyl-cinnamyl-hydrazono)-propionate, glucose utilization increased from 0.276 ± 0.043 μηιοί · g"1 · I""1 to 0.894 + 0.303 μιηοΐ · g"1 · Γ1 (ρ < 0.05). Pyruvate and lactate utilization were stimulated to a lesser extent, while acetate utilization remained nearly constant. At a concentration of 2 mmol/1 2-(3-methyl-cinnamyl~hydrazono)-propionate, the oxidation of palmitate decreased from 0.214 ± 0.017 μηιοί. g-i. j-i to 0.060 ± 0.005 μηιοί · g"1 · I"1, while the oxidation of octanoate was not decreased. These findings point to a stimulation of the glycolytic flux by inhibition of long-chain fatty acid oxidation