Figure S4. NMR Chemical Shift Perturbations in Response to dsDNA Binding to Mcm10

Stereo diagrams of two representative sections of the final refined model (gold sticks) are shown superimposed onto composite omit electron density maps contoured at 1σ. (A) α-helical region Asn261-Glu284. (B) Antiparallel β-sheet formed from strands β1, β4, and β5.2 (arrows). Figure S2. NMR Chemical Shift Assignments and Perturbation by ssDNA Binding (A) 1H-15N HSQC spectrum of Mcm10-ID with assigned chemical shifts labeled by residue number. (B) 1H-15N HSQC spectrum for Mcm10-ID in the absence (black) and presence (red) of ssDNA. The spectrum of the complex was acquired at a protein:DNA ratio of 1:1. The region of the spectra shown in Figures 4 and S4 is boxed