Phenotypingof Lipemias by Ultrafiltrationand Nephelometryof Serum Lipoproteins

Nephelometry of serum before and after ultrafiltrationthrough cellulose membranes affords a simple and rapid physical method for separately estimating pre..lipoproteins and chylomicrons in their native state. The method was rigorouslyevaluated through correlativestudies with chemical analyses, electron microscopy, and electrophoresis. Together, the data indicate that this technique, combined with the determination of choles-terol as an estimate of-lipoprotein concentration, allows the pheno-typing of hyperlipemias. Additional Keyphrases electron microscopy of lipoproteins #{149} latex particles as size indices #{149} sulfo-phospho-vanillin method for total lipids choles!erol and lriqlycerides methodological correlations #{149} /3- and pre-/3-lipoproteins chyloinicrons particle-size--filter-pore diameter relationship C HEMICAL METHODS OF lipid analysis usually destroy lipoprotein complexes in which lipids exist in serum. Lipoprotein analysis, therefore, is usually based on physical principles. Electro-phoresis is commonly used, but differences in dye affinity among lipoprotein classes present certain difficulties to quantitation. This paper evaluates nephelometry of pre-,3-lipoproteins and chylo-microns after they have been separated by ultra-filtration. Lipoproteins remain in their native state throughout the procedure