Studies on Serum Lipoprotein 2. Electropaperchromatography of lipoprotein especially the masked lipid

By using the apparatus demonstrated in the first report, the author has studied the lipoprotein of serum, extending at 110 v. for 7 hours filling the space with air and staining with Sudan black B (S. B. B). The following results have been obtained. 1. From the integrated values of the area obtained by densitometry on the papers stained with S. B. B it is possible to know roughly the amount of lipoprotein of serum and exact quantitative relation between α and β lipoproteins. 2. The drying of paper causes the masking of the lipids of lipoprotein which can be unmasked again by heating at 110℃. for 20 minutes. 3. Pepsin, phenol and some carboxylic acids act as to unmask the masked lipoprotein. After unmasking some lipids escape into the solvent during the S. B. B staining in α-lipoprotein, but not in β-lipoprotein. 4. From the observations on the masking and unmasking of serum lipoproteins the binding modus between lipid and protein and the masking-unmasking mechanisms have been discussed