Effect of lsoenzyme Composition on Kodak Ektachem Test Results for Creatine Kinase, Lactate Dehydrogenase

Seventy-four patients ’ sera containing macroenzymes or other uncommon enzyme forms were analyzed for total creatine kinase, lactate dehydrogenase, alkaline phospha-tase, or amylase activity on an Ektachem 700XR analyzer. The Ektachem test results correlated well with activities obtained by means of liquid reagents on RA-1 000. For each enzyme tested, linear-regression analysis yielded data com-parable with those for sera containing exclusively the more common isoenzymes. Increased concentrations of intestinal ALP, however, resulted in higher activities of total ALP being reported for the Ektachem. Additional Keyphrases: multilayer film analysis. macroenzymes mitochondrialenzymes. enzyme-immunoglobulin complexes During the past decade, the Ektachem multilayer film technology (Eastman Kodak, Rochester, NY) has becom