Two-Dimensional Immunoelectrophoretic Pattern of Low- and Very-Low-Density Lipoproteins,with ParticularReference to Fredrickson’s Type Ill

I report the pattern of low-density lipoproteins (LDL) and very-low-density lipoproteins (VLDL) as revealed by two-dimensional (crossed) immunoelectrophoresls in hyper-lipoproteinemia types II, Ill, and IV, preliminarily diagnosed by means of a complex ilpoprotein profile examination. The higher affinity of Oil Red stain for VLDL- than for LDL-immunoprecipitation zones is peculiar to this lipo-protein class, and crossed immunoelectrophoretograms may thus be helpful for laboratory diagnosis of types lIb and IV In problematic cases. The position and the medium affinity for Oil Red of “broad beta ” zones appearing In immunoelectrophoretograms of type Ill hyperlipopro-teinemia are suggested as useful criteria for the diagnosis of this condition. From the structural and staining char-acteristics of VWL-immunoprecipitation zones, alteration of both composition and antigenic properties of VLDL in hyperlipoproteinemias lIb-IV is deduced. I present in detail the procedure for crossed immunoelectrophoresis as well as that for the lipoprotein profile examination used in this study. AdditIonal Keyphrases: hyperlipoproteinemia. lipoproteins #{149}diagnostic aids ‘ heart disease The mobility of a protein molecule in an electrical field primarily depends on the balance between its positive and negative charges at the electrophoretic pH value. The electrophoretic properties of a lipoprotein also appear to be largely determined by the charge on its apoprotein moiety; any additional charge contribu-tion from phospholipids that do not exist in form of zwitterions at the given pH (1) seems to be negligible