Natural Killer Cell Activity and Macrophage-Dependent Inhibition of Growth or Killing of Mycobacterium avium Complex in a Mouse Model

Natural killer (NK) cells from spleens of normal and Mycobacterium avium complex (MAC)-infected C57 black mice (C57 BL/6 bg/+) were examined for their capacity to activate splenic and peritoneal macrophages from beige mice to inhibit or kill intracellular MAC. Peritoneal and splenic macrophages from beige mice were exposed in vitro to NK cells obtained from MAC-infected and uninfected black mice. NK cells from uninfected black mice were also treated in vitro with recombinant interleukin-2 (IL-2) for 48 h before incubation with macrophages. While control macrophages supported intracellular growth of MAC, macrophages exposed to unactivated NK cells inhibited growth of the intracellular bacteria, as determined 4 days after infection. IL-2 stimulated NK cells, and NK cells obtained from MAC-infected animals were able to activate murine macrophages in vitro to inhibit growth or kill 40.0 ± 5 % and 61.3 ± 6 % of the intracellular bacteria, respectively. In other experiments, beige mice (C57 BL/6 bg/bg) were treated intraperito-neally with NK cells obtained from MAC-infected and uninfected C57 black mice. Perito-neal macrophages harvested from beige mice treated with NK cells activated in vitro with IL-2 killed 24.4 ± 4 % of Intracellular bacteria by day 4 after infection. Macrophages ob-tamed from animals treated with NK cells harvested from MAC-infected black mice killed 58.8 ± 7 % of intracellular bacteria by 4 days after infection, in contrast with intracellular growth observed in macrophages obtained from untreated animals and from animals treated with Hanks ’ solution or unactivated NK cells. These crossover studies suggest that NK cells may be important in host defense against MAC. Key words: tumor necrosis factor, interferon-gamma, intracellular bacteri