The Inhibition Activity of Extracts from Fontinalis Antipyretica and β-carboline Alkaloids on Enzymes Acetylcholinesterase and Butyrylcholinesterase

The inhibition activity of extracts from Fontinalis antipyretica and β-carboline alkaloids on enzymes acetylcholinesterase and butyrylcholinesterase This thesis deals with testing of inhibition activity of methanolic and acetonic extracts from water moss Fontinalis antipyretica (Fontinalaceae) and β-carboline alkaloids on activity of enzymes acetylcholinesterase (ACHE) and butyrylcholinesterase (BUCHE) using test "Fast Blue B salt" at TLC desk and Ellman's test using spectrophotometer. It was also investigated how dimethylsulfoxide used as a solvent in combination with water affects activity of enzymes and alkaloids. Another part of this work is focused on discovering in which locality water moss occures and what kind and quantity of β-carboline alkaloids contains. For this evaluation was applicated HPLC analysis. Results show that better inhibition activity of extracts on ACHE and BUCHE has acetonic extract whereas the hightest activity show acetonic extract on BUCHE. Harmine in form of base and salt in water and in mixture of DMSO and water has the hightest inhibition activity on ACHE using eserine as reference substance. Harmalol in form of salt in water and harmine in form of base and salt in mixture of DMSO and water has the hightest activity on BUCHE. It was find out that DMSO considerably..